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目的探讨山东省汉坦病毒(HV)的分子流行病学特点。方法用反转录-聚合酶链反应(RT-PCR)及核苷酸序列测定技术,对肾综合征出血热(HFRS)监测点的阳性鼠肺标本进行目的基因扩增并测序,与国内外的HV毒株进行同源性分析及系统发生树分析。结果有15份标本扩增出汉城(SEO)型S、M片段,对其中10份扩增片段进行序列分析认为均为SEO型HV,S片段之间的同源性≥96.3%,其推导的氨基酸序列同源性(JN5-153S和DY1S除外)≥96.8%;M片段之间及与山东省以往分离的ZB8同源性≥97.5%,其推导的氨基酸序列同源性为98.6%～100%。结论近年来山东省流行的HV仍以SEO型S3亚型为主。同一地区或地理位置相邻的地区病毒基因组同源性较高,具有明显的地区聚集性,基因型别较为稳定。
Objective To investigate the molecular epidemiological characteristics of Hantavirus (HV) in Shandong Province. Methods RT - PCR and nucleotide sequencing were used to amplify and sequence the positive lung samples of hemorrhagic fever with renal syndrome (HFRS) Homology analysis and phylogenetic tree analysis of HV strains. Results Seventy-two samples of S / M of SEO were amplified, and 10 of them were sequenced and all were SEO-type HV. The homology between S fragments was ≥96.3%. The deduced The amino acid sequence homology (excluding JN5-153S and DY1S) was ≥96.8%. The homology between M fragments and ZB8 previously isolated in Shandong Province was ≥97.5%, and the deduced amino acid sequence homology was 98.6% -100% . Conclusion In recent years, the prevalence of HV in Shandong is still dominated by the SEO type S3 subtype. The same region or geographical proximity of the virus genome homology is high, with a significant regional aggregation, genotypes are more stable.