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谷胱甘肽S-转移酶(GST,E.C.2,5,1,18)是一类分布广泛的多功能蛋白,有多种同工酶形式,在大鼠中至少已发现12种,均为二聚体,分碱性、中性和酸性三大类。肝脏中GST的含量和种类最多,大多为碱性型。GST参与致癌剂的结合和解毒,又发现胎盘型GST(在大鼠中称GST-P或GST7-7,为胎盘中的唯一形式)能在早期肝癌中表达,是一个癌前病变的酶学标志。本文用Solt和Farber制订的顺序诱发大鼠肝癌,研究其早期病变中总GST活力及GST-P含量的变化,并用免疫组织化学方法观察了GST-P在肝组织中的定位。
Glutathione S-transferase (GST,EC2,5,1,18) is a class of widely distributed multifunctional proteins with multiple isoenzyme forms. At least 12 species have been found in rats. Dimer, divided into three major categories of alkaline, neutral and acidic. The content and type of GST in the liver are the most, mostly alkaline. GST is involved in the combination and detoxification of carcinogens, and it has also been discovered that placental GST (GST-P or GST7-7, the only form in the placenta in rats) can be expressed in early liver cancer and is an enzymology of precancerous lesions. Sign. In this study, Solt and Farber developed the sequence to induce rat liver cancer, to study the changes of total GST activity and GST-P content in early stage lesions, and to observe the localization of GST-P in liver tissue by immunohistochemistry.