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吡喹酮具有预防小鼠感染血吸虫尾蚴的作用,表现为既能杀死侵袭宿主皮肤的血吸虫尾蚴,又能杀死侵入宿主皮肤内的童虫(乐文菊1985,肖树华1986,中国药理学报).本文报道吡喹酮对已侵入小鼠皮肤组织内的日本血吸虫童虫的作用。 方法 药物吡喹酮溶解于聚乙二醇(PEG 400)中,浓度为40～60mg/ml。 光学显微镜观察取18～22g的雌小鼠,每鼠经腹部皮肤感染300～400条日本血吸虫尾蚴,3h后给服吡喹酮400mg/kg。给药后0.5、2及24h,取接种部位皮肤收集童虫,滴加0.03％美蓝后,在光学显微镜下观察虫体的形态变化及虫体着色情况;或将皮肤固定于10％福尔马林中,制备厚度为7μm的石
Praziquantel has the effect of preventing mice from infecting cercariae of Schistosoma japonicum by killing both the schistosome cercariae that invade the skin of the host and the schistosomiasis that invades the skin of the host (Le Wenju 1985, Xiao Shuhua 1986, Chinese Pharmacological Bulletin) This article reports the effect of praziquantel on Schistosoma japonicum, a member of the skin tissue that has invaded mice. Method Praziquantel was dissolved in polyethylene glycol (PEG 400) at a concentration of 40-60 mg / ml. Light microscopic observation Take 18 ~ 22g of female mice, each mouse infected by abdominal skin 300 to 400 cercariae Schistosoma japonicum, 3h after taking praziquantel 400mg / kg. At 0.5, 2 and 24 h after the administration, the mice were collected from the skin of the inoculation site, and the morphology of the parasites and the color of the parasites were observed under a light microscope after dropping 0.03% methylene blue. The skin was fixed on a 10% Marlin, a stone having a thickness of 7 μm was prepared