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目的了解反复高 +Gz暴露后大鼠脑组织中IL 1 β和TNF αmRNA表达变化。方法 2 0只SD大鼠随机分成对照组、+Gz重复暴露后 3 0min、6h、2 4h和 48h五组 ,每组 4只。对照组大鼠G值为 +1Gz ,实验组大鼠在动物离心机上经历了 3次 +1 4Gz/45s(两次间间隔 3 0min)作用。分别于暴露后 3 0min、6h、2 4h和 48h处死大鼠取脑 ,提取总RNA ,用半定量反转录聚合酶链反应 (RT -PCR)检测方法检测大鼠脑组织中IL 1 β和TNF αmRNA表达水平。结果 +Gz暴露后 3 0min、6h和 2 4h大鼠脑组织IL 1 β和TNF αmRNA均有升高 ,但 48h时均恢复正常。结论反复高 +Gz暴露可刺激大鼠脑组织内IL 1 β和TNF αmRNA表达 ,它们在 +Gz所致脑损伤中起一定的作用 ,但这种变化是可逆的
Objective To investigate the changes of IL-1β and TNFα mRNA expression in rat brain after repeated high + Gz exposure. Methods Twenty SD rats were randomly divided into control group, Gz repeated exposure 30min, 6h, 24h and 48h five groups with 4 rats in each group. The G value of the control group was + 1Gz, and the rats in the experimental group underwent three cycles of +1 4Gz / 45s on the animal centrifuge (with an interval of 30 minutes between the two groups). The rats were sacrificed at 30 min, 6 h, 24 h and 48 h after exposure respectively. The total RNA was extracted and the levels of IL-1β and IL-1β were determined by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) TNF α mRNA expression levels. Results The levels of IL 1 β and TNF α mRNA increased at 30 min, 6 h and 24 h after + Gz exposure, but returned to normal at 48 h. Conclusions Repeated high + Gz exposure can stimulate the expression of IL-1β and TNFα mRNA in brain tissue of rats, which play a role in + Gz-induced brain injury, but this change is reversible