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目的：研究表皮生长因子受体基因对于胶质瘤发生发展的作用，探索以ＥＧＦＲ反义ＲＮＡ进行反义基因治疗胶质瘤的可行性。方法：将含ＥＧＦＲ反义ＲＮＡ的质粒通过脂质体介导转入Ｃ６恶性胶质瘤细胞，通过Ｓｏｕｔｈｅｒｎ印迹杂交鉴定外源基因整合情况，ＭＴＴ法测定细胞存活率，Ｎｏｒｔｈｅｒｎ印迹杂交、细胞原位ｍＲＮＡ杂交及ＥＧＦＲ免疫组化染色检测ＥＧＦＲｍＲＮＡ及蛋白表达，核仁组成区相关嗜银蛋白染色（ＡｇＮＯＲ计数）检测细胞增殖活性。结果：Ｓｏｕｔｈｅｒｎ印迹杂交表明外源性反义ＥＧＦＲ基因（互补于ＥＧＦＲ基因全长及３端部分序列）分别在Ｃ６细胞中整合（分别命名为Ｃ－ｐＲ１和Ｃ－ｐＲ３），通过Ｎｏｒｔｈｅｒｎ印迹杂交、原位杂交及细胞免疫组织化学检测均显示转染ＥＧＦＲ反义ＲＮＡ的Ｃ６细胞比转染前ＥＧＦＲｍＲＮＡ水平及ＥＧＦＲ蛋白水平有不同程度的降低。表达高水平反义ＲＮＡ的克隆在培养状态下细胞增殖活性较对照组有明显下降。结论：ＥＧＦＲ在恶性胶质瘤细胞的发生发展过程中起十分关键的作用，ＥＧＦＲ有可能成为基因治疗恶性胶质瘤的优选靶的之一。
Objective: To investigate the role of epidermal growth factor receptor gene in the development of gliomas and explore the feasibility of using EGFR antisense RNA for antisense gene therapy of glioma. Methods: The plasmid containing EGFR antisense RNA was transfected into C6 malignant glioma cells by liposome, the integration of foreign genes was identified by Southern blot hybridization, the cell viability was determined by MTT assay, Northern blot hybridization, cell in situ The expression of EGFR mRNA and protein was detected by mRNA hybridization and EGFR immunohistochemical staining. AgNOR count was used to detect the cell proliferation activity. RESULTS: Southern blot hybridization showed that the exogenous antisense EGFR gene (complementary to the full-length and 3 -terminal part of the EGFR gene) was integrated in C6 cells (designated as C-pR1 and C-pR3, respectively) and hybridized by Northern blotting. In situ hybridization and immunohistochemistry showed that C6 cells transfected with EGFR antisense RNA had lower levels of EGFR mRNA and EGFR protein levels before transfection. Colonies expressing high levels of antisense RNA had a significantly decreased cell proliferation activity compared to the control group. Conclusion: EGFR plays a key role in the development of malignant glioma cells. EGFR may be one of the preferred targets for gene therapy of malignant gliomas.