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为给霍乱弧菌亚单位菌苗的研制提供基础，作者对霍乱弧菌Ｏ１３９菌毛的提取和鉴定方法进行了探讨。结果发现，菌毛的最佳表达条件为ＡＫＩ或ＣＦＡ培养基中３０℃静止培养２４～３６小时。ＳＤＳ－ＰＡＧＥ电泳证实了ＴｃｐＡ蛋白的存在，分子量为２０．５ｋｄ。用鼠抗毒素共调菌毛（ＴＣＰ）的单克隆抗体和羊抗鼠ＩｇＧ－ＨＲＰ作斑点酶免疫试验，结果为阳性。用ＴＣＰ免疫大白兔所获得的免疫血清与Ｏ１３９型和ＥｌＴｏｒ型霍乱弧菌菌株作常规凝集试验，可见ＴＣＰ与Ｅ１Ｔｏｒ型菌株发生交叉反应。由此表明ＴＣＰ可作为一种有效的保护抗原，用于霍乱菌苗的研制
To provide a basis for the development of Vibrio cholera subunit vaccine, the author explored the extraction and identification of V. cholerae O139 pili. As a result, it was found that the optimal conditions for expression of pilus were static culture in AKI or CFA medium at 30 ° C for 24 to 36 hours. SDS-PAGE electrophoresis confirmed the existence of TcpA protein, molecular weight of 20.5kd. Monoclonal antibodies against goat anti-toxin co-pili (TCP) and goat anti-mouse IgG-HRP were used as the dot blot immunoassay and the result was positive. Conventional agglutination test was performed on the immune sera obtained from the immunized rabbits with TCP and strains of Vibrio cholerae O139 and ElTor, showing that TCP cross-reacts with strain E1Tor. This indicates that TCP can be used as an effective protective antigen for the development of cholera vaccine