用改进的凝胶扩散法对国内外 5 7个春小麦品种 (系 )在灌浆后 35d进行了穗发芽抗性和α 淀粉酶活性的测定。结果表明 :不同春小麦品种的穗发芽抗性及α 淀粉酶活性存在显著差异。来自黑龙江省的大部分主栽品种的穗发芽抗性中等 ,红粒品种的穗发芽抗性比普通白粒品种强 ,在白粒品种中也存在抗性极强的品种。穗发芽率与种子中α 淀粉酶活性存在显著正相关 ,α 淀粉酶活性可作为鉴定穗发芽抗性的生化指标 Sprouting resistance and α-amylase activity of 57 spring wheat varieties (lines) at home and abroad were tested by modified gel diffusion method 35 days after grouting. The results showed that there were significant differences in sprouting resistance and α-amylase activity among different spring wheat cultivars. Most of the main cultivars from Heilongjiang province had moderate sprouting resistance, and their resistance to sprouting was stronger than that of common white cultivars. There were also highly resistant cultivars among cultivars. There was a significant positive correlation between spike germination percentage and α-amylase activity in seed, and α-amylase activity could be used as biochemical index to identify sprout germination resistance