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在研究大鼠肝癌变过程中氨甲酰磷酸合成酶I(CPS_1)活性降低机制同时,作者自大鼠肝线粒体中,经十六烷基三甲基溴化铵处理,硫酸铵分步沉淀及QAE-Sephadex A-50柱层析,分离提纯了CPS_1。本法能将CPS_1从尿素循环中另一线粒体酶鸟氨酸氨甲酰转移酶(OCT)中全部析出。纯化的 CPS_1制剂在聚丙烯酰胺凝胶电泳中呈均一状。由纯化的CPS_1制备的单价兔抗血清可以完全抑制正常大鼠肝及二乙基亚硝胺(DENA)诱发肝癌中的CPS_1
In the study of rat liver carcinogenesis carbamoyl-phosphate synthetase I (CPS 1) activity decreased at the same time, the author from rat liver mitochondria, cetyl trimethyl ammonium bromide treatment, step-by-step precipitation of ammonium sulfate and QAE-Sephadex A-50 column chromatography, separation and purification of CPS_1. This method is able to completely precipitate CPS_1 from the other mitochondrial enzyme Ornithinecarbamyltransferase (OCT) in the urea cycle. Purified CPS 1 preparations were homogenous in polyacrylamide gel electrophoresis. Monovalent rabbit antiserum prepared from purified CPS_1 could completely inhibit CPS_1 induced by normal rat liver and DENA