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目的从血管生成的角度探讨芪棱汤抗大鼠脑缺血再灌注损伤的机理。方法将大鼠随机分为假手术组、模型组、芪棱汤去养阴药组及芪棱汤组。采用颈内动脉线栓法复制大鼠脑缺血再灌注模型。在再灌注不同时点采用免疫组织化学法测VEGF;CD34标记微血管计数。结果假手术组VEGF表达极弱;除缺血再灌注6h外,各相应再灌注时间点12h、1d、3d、7d,芪棱汤组及芪棱汤去养阴药组较模型组的VEGF表达明显增多,且芪棱汤组与芪棱汤去养阴药组有显著差异。CD34标记微血管计数再灌注各时间点芪棱汤组、芪棱汤去养阴药组与模型组比较均有显著差异;芪棱汤组与芪棱汤去养阴药组有显著差异。结论芪棱汤通过促进VEGF表达的上调,促进微血管新生,可能是其抗脑缺血再灌注损伤的作用机制之一。
Objective To investigate the mechanism of Qileng decoction on cerebral ischemia-reperfusion injury in rats from the perspective of angiogenesis. Methods The rats were randomly divided into sham-operated group, model group, Qi-Ling-Tang nourishing yin-yin group and Qileng Decoction group. The rat model of cerebral ischemia-reperfusion was reproduced by the method of internal carotid artery occlusion. At different time points after reperfusion, VEGF was detected by immunohistochemistry; CD34 labeled microvessel count. Results The expression of VEGF in sham operation group was very weak. In addition to ischemia-reperfusion 6h, the respective reperfusion time points were 12h, 1d, 3d, 7d. The expression of VEGF in Qilngtang group and Qilingng decoction group was higher than that in model group Significantly increased, and Qileng Decoction group and Qiluan Decoction yin ying group were significantly different. CD34 labeled microvessel count reperfusion at various time points Qiluan Tang group, Qilng Decoction yin yin group compared with the model group were significantly different; Qiliantang group and Quling Decoction yin yao group were significantly different. Conclusion Qilian Decoction can promote angiogenesis by up-regulating the expression of VEGF, which may be one of the mechanisms of its anti-cerebral ischemia-reperfusion injury.