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目的:采用淋巴管特异性标志物podoplanin标记口腔鳞癌组织和正常口腔组织中的淋巴管,并计数淋巴管密度(lymphatic vessel density,LVD)值,探讨口腔鳞癌及癌周淋巴管密度与淋巴结转移的相关性及可能机制。方法:采用免疫组织化学方法检测21例正常口腔黏膜组织和88例口腔鳞癌患者组织的podoplanin表达;用podoplanin标记淋巴管,并计数口腔鳞癌和癌周组织的淋巴管密度。采用SPSS13.0软件包对数据进行t检验,分析正常组织和癌组织、淋巴结转移组和未转移组的淋巴管密度(癌周、癌内)。结果:口腔鳞癌及癌周组织的淋巴管密度均高于正常口腔黏膜组织,有显著差异(P<0.05)。癌组织内淋巴管小而闭锁,癌组织周围的淋巴管大而扩张;淋巴结转移组的癌周淋巴管密度(14.270±4.610)显著高于无转移组(9.450±2.411),差异具有显著性(P<0.05),癌组织淋巴管密度在2组间无显著差异。结论:口腔鳞癌患者癌周淋巴管的生成可能是影响区域淋巴结转移的重要因素。
OBJECTIVE: To detect lymphatic vessel density (LVD) in oral squamous cell carcinoma and normal oral squamous cell carcinoma using podoplanin, a lymphatic vessel specific marker, and to investigate the relationship between lymphatic vessel density (LVD) and oral squamous cell carcinoma Relevance of the transfer and possible mechanisms. Methods: Immunohistochemical method was used to detect the expression of podoplanin in 21 cases of normal oral mucosa and 88 cases of oral squamous cell carcinoma. The lymphatic vessels were labeled with podoplanin and the lymphatic vessel density of oral squamous cell carcinoma and peri-cancerous tissues were counted. Data were analyzed by t-test using SPSS 13.0 package to analyze the lymphatic vessel density (pericancerous and intracranial) in normal and cancerous tissues, lymph node metastases and non-metastatic groups. Results: The density of lymphatic vessels in oral squamous cell carcinoma and pericancerous tissues were significantly higher than that in normal oral mucosa (P <0.05). The lymphatic vessels in the cancer tissues were small and atresia, and the lymphatic vessels around the cancer tissues were enlarged. The lymphatic vessel density in the lymph node metastasis group (14.270 ± 4.610) was significantly higher than that in the non-metastasis group (9.450 ± 2.411), the difference was significant P <0.05). There was no significant difference in lymphatic vessel density between the two groups. Conclusion: The generation of lymphangiogenesis in patients with oral squamous cell carcinoma may be an important factor affecting the regional lymph node metastasis.