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目的探讨血管内皮生长因子(VEGF)、细胞周期期素1(CyclinD1)在乳腺癌中的表达,分析其在乳腺癌发生、发展中的作用及二者与乳腺癌临床各参数间的关系。方法应用SP免疫组化法检测VEGF和CyclinD1在60例乳腺癌、21例非典型增生、10例正常对照组石蜡包埋组织中的表达。结果 VEGF、CyclinD1的表达在乳腺癌组、非典型增生组及正常对照组间差异有统计学意义(P<0.01);轻度非典型增生组与中重度非典型增生组间VEGF阳性表达差异具有统计学意义(P<0.05)。VEGF的阳性表达与年龄、肿瘤大小、组织学分级、临床分期、腋淋巴结转移情况、ER、PR情况无关(P>0.05)。CyclinD1的表达与腋淋巴结转移情况有关。乳腺癌组织VEGF和Cyclind1两者表达呈正相关(r=0.289,P<0.05)。结论 VEGF和CyclinD1在非典型增生及乳腺癌中的异常表达提示其与肿瘤的发生、发展密切相关,联合检测VEGF和CyclinD1有可能成为乳腺癌早期诊断及预后的重要指标。
Objective To investigate the expression of vascular endothelial growth factor (VEGF) and cyclinD1 in breast cancer and its relationship with the occurrence and development of breast cancer and their relationship with clinical parameters of breast cancer. Methods SP immunohistochemistry was used to detect the expression of VEGF and CyclinD1 in 60 cases of breast cancer, 21 cases of atypical hyperplasia and 10 cases of normal control. Results The expressions of VEGF and CyclinD1 in breast cancer group, atypical hyperplasia group and normal control group were significantly different (P <0.01). The difference of VEGF positive expression between mild dysplasia group and moderate-severe atypical hyperplasia group was Statistical significance (P <0.05). The positive expression of VEGF had no correlation with age, tumor size, histological grade, clinical stage, axillary lymph node metastasis, ER, PR (P> 0.05). CyclinD1 expression and axillary lymph node metastasis. The expression of VEGF and Cyclind1 in breast cancer tissues was positively correlated (r = 0.289, P <0.05). Conclusions The abnormal expression of VEGF and CyclinD1 in atypical hyperplasia and breast cancer suggests that it is closely related to the occurrence and development of tumor. Combined detection of VEGF and CyclinD1 may be an important index for early diagnosis and prognosis of breast cancer.