临床静脉注射IL-2时会产生明显的毒副作用,口服IL-2作为一种替代途径有望解决这一难题,但目前尚不清楚口服IL-2是否具有全身效应.为此作者通过给小鼠口服IL-2来检测其对外周血白细胞数和骨髓增殖的影响,来判断其是否产生全身效应.以6～8周龄G57BL/6小鼠作为实验动物,实验组分为四组,分别口服经明胶稀释的不同浓度的IL-2(40,400,4000,40000U/d);阳性对照组皮下注射经BSA和PBS稀释的IL-2(5000U/d),阴性对照组口服明胶稀释液或皮下注射BSA和PBS稀释液,连续给药三天后检测结果表明,口服IL-2(40,400,4000,40000U/d)的实验组,其外周血白细胞数分别降至未给药前加93.7±1.3%,83.6±2.6%,75.0±1.8%;75.4±2.3%;阳性对照组降至实验前的71.7±1.8%,而阴性对照组无明显差异.这表明小鼠外周血白细胞数随IL-2口服剂量的增加而降低.由于外周血白细胞数受骨髓功能的影响,为此进一步检测了小鼠口服IL-2后的骨髓功能.实验组口服IL-2(4000U/d)或皮下注射IL-2(5000U/d),阴性组口服明胶稀释液或皮下注射BSA和PBS稀释液,连续给药三天后杀死小鼠,取出骨髓细胞在含不同浓度M-CSF的培养基中培养七天,计算GM克隆数,测得在同一浓度加M-CSF中实验组得到的GM克隆数要少于对照组,说明口服的IL-2是通过抑制骨髓细胞的增殖来降低外 Clinical intravenous injection of IL-2 will have obvious side effects, oral administration of IL-2 as an alternative route is expected to solve this problem, but it is not clear whether oral administration of IL-2 has systemic effects.To this end, the author of mice Oral administration of IL-2 to test its effect on peripheral blood leukocyte count and myeloid proliferation to determine whether it has systemic effects.With 6 to 8 weeks old G57BL / 6 mice as experimental animals, the experimental group was divided into four groups, oral (40,400,4000,40000U / d) with different concentrations of gelatin. The positive control group was subcutaneously injected with IL-2 (5000U / d) diluted in BSA and PBS, the negative control group was given gelatin dilution or subcutaneous injection BSA and PBS, the results of three days after continuous administration showed that the number of peripheral blood leukocytes in the experimental group orally administered with IL-2 (40,400,4000,40000U / d) decreased to 93.7 ± 1.3% before administration, respectively, 83.6 ± 2.6%, 75.0 ± 1.8% and 75.4 ± 2.3%, respectively.The positive control group dropped to 71.7 ± 1.8% before the experiment, but there was no significant difference between negative control group.This indicated that the number of peripheral blood leukocyte in mice increased with the oral dosage of IL-2 Due to the influence of the number of peripheral white blood cells by bone marrow function, to further test the mice oral IL -2 after the administration of IL-2 (4000U / d) or subcutaneously injected IL-2 (5000U / d), the negative group of oral administration of gelatin dilution or subcutaneous injection of BSA and PBS diluent, continuous administration of three days The mice were sacrificed and the bone marrow cells were removed and cultured in medium containing different concentrations of M-CSF for seven days to calculate the number of GM colonies. It was found that the number of GM colonies obtained in the experimental group at the same concentration plus M-CSF was less than that of the control group, Oral IL-2 is shown to be reduced by inhibiting the proliferation of myeloid cells