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目的探讨T2DM患者血浆中瘦素、胰岛素水平及其microRNA(miRNA)表达的相关性研究。方法选取150例T2DM患者(T2DM)和150名NGT者,比较两组瘦素、胰岛素等水平。瘦素和胰岛素水平采用RIA kit法测定。采用mirVana~(TM)PARIS~(TM)Kit提取血浆中RNA,采用TaqMan标准的RT-PCR分析测定miRNA-296及miRNA-9的含量。结果 miRNA-296表达与血浆中瘦素水平有相关性,miRNA-9表达与胰岛素水平有相关性。结论 T2DM患者血浆中瘦素、胰岛素水平及其miR NA表达有相关性,但机制尚不明确。
Objective To investigate the correlation between plasma leptin, insulin levels and microRNA (miRNA) expression in T2DM patients. Methods A total of 150 T2DM patients and 150 NGT patients were selected. The levels of leptin and insulin in the two groups were compared. Leptin and insulin levels were measured using the RIA kit method. RNA was extracted from plasma using mirVana ~ (TM) PARIS ~ (TM) Kit, and the contents of miRNA-296 and miRNA-9 were determined by TaqMan standard RT-PCR analysis. Results There was a correlation between the expression of miRNA-296 and plasma leptin level, and the correlation between miRNA-9 expression and insulin level. Conclusions Plasma leptin and insulin levels and the expression of miR-NA in T2DM patients are correlated, but the mechanism is still unclear.