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目的探讨肌肽二肽酶1(CNDP1)基因多态性与中国汉族人群糖尿病慢性肾脏疾病(CKD)的关系。方法选取健康对照者(NC)90名、T2DM患者210例和CKD患者280例(CKD组再分为GFR≥90 ml/(min·1.73 m~2)亚组105例,60 ml/(min·1.73 m~2)≤GFR<90 ml/(min·1.73 m~2)亚组84例,GFR<60 ml/(min·1.73 m~2)亚组91例),利用聚合酶链反应限制性片段长度多态性分析法(PCR-RFLP)行CNDP1 rs4892247位点多态性检测。结果 NC、T2DM和CKD组TT、CT、CC 3种基因型频率分别为66.7%、26.7%、6.7%,60.5%、31.9%、7.6%和46.8%、43.2%、10.0%;C等位基因频率分别为20.0%、23.6%、31.6%;NC组与CKD组,T2DM组与CKD组基因型和等位基因C的频率分布差异有统计学意义(P<0.05)。Logistic回归分析显示,携带C等位基因的CNDP1与CKD的发生呈正相关(OR=2.634,P<0.05)。结论 CNDP1 rs4892247基因多态性可能与中国汉族人群发生CKD发病风险增高相关。
Objective To investigate the relationship between CNDP1 polymorphism and chronic kidney disease (CKD) in Chinese Han population. Methods Ninety healthy control subjects (NC), 210 T2DM patients and 280 CKD patients (CKD group were divided into 105 subgroups of GFR≥90 ml / (min · 1.73 m ~ 2), 60 ml / (min · 84 cases in subgroup and 91 cases in subgroup of GFR <60 ml / (min · 1.73 m ~ 2) in subgroup of 1.73 m ~ 2 ≤GFR ≤ 90 ml / (min · 1.73 m ~ 2). Polymerase chain reaction restriction The polymorphism of CNDP1 rs4892247 was detected by PCR-RFLP. Results The frequencies of TT, CT and CC genotypes in NC, T2DM and CKD groups were 66.7%, 26.7%, 6.7%, 60.5%, 31.9%, 7.6% and 46.8%, 43.2% and 10.0% The frequencies of genotype and allele C in NC group, CKD group, T2DM group and CKD group were significantly different (P <0.05). Logistic regression analysis showed that there was a positive correlation between CNDP1 carrying C allele and CKD (OR = 2.634, P <0.05). Conclusion The polymorphism of rs4892247 in CNDP1 may be related to the increased risk of CKD in Chinese Han population.