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采用直线加速器产生3.60×1011、7.10×1011和3.54×1012/cm2 3种辐照注量的快中子处理肇东苜蓿干种子,对其进行种子发芽试验、幼苗生长和RAPD分子标记分析。研究表明,经3种注量快中子处理后,肇东苜蓿种子发芽势和发芽率显著高于对照;幼苗的苗高和根长小于对照,随着快中子处理注量的增加,幼苗苗高降低和根长减少的幅度越大;当快中子辐照注量达到3.54×1012/cm2时,幼苗根长相比对照降低了81.63%,没有生长出真叶,只有子叶。RAPD试验共使用36种引物,结果显示,辐照注量为3.60×1011、7.10×1011和3.54×1012/cm2的快中子处理紫花苜蓿M1代的RAPD多态性频率分别为7.25%、6.52%和5.80%,3.60×1011/cm2处理辐照注量的RAPD多态性频率最高。在本研究的辐照注量范围内,3.60×1011/cm2是利用快中子诱变紫花苜蓿的适宜辐照注量。
Fast linseed seeds of Zhaodong alfalfa were treated with linacs of 3.60 × 1011, 7.10 × 1011 and 3.54 × 1012 / cm2 by linear accelerator, and germinated seeds, seedling growth and RAPD markers were analyzed. The results showed that seed germination potential and germination rate of alfalfa alfalfa were significantly higher than that of the control after three fast-fluence neutron treatments. Seedling height and root length of alfalfa were lower than those of the control. With the increase of fast neutron flux, When the fast-neutron irradiation fluence reached 3.54 × 1012 / cm2, the seedling root length decreased by 81.63% compared with the control, no true leaves were grown, and only cotyledons were present. A total of 36 primers were used in the RAPD test. The results showed that RAPD frequencies of fast neutron-fed alfalfa M1 generation with irradiation doses of 3.60 × 1011, 7.10 × 1011 and 3.54 × 1012 / cm2 were 7.25% and 6.52 % And 5.80%, 3.60 × 1011 / cm2 irradiation frequency of RAPD polymorphism the highest frequency. In this study, the irradiation dose range, 3.60 × 1011 / cm2 is the use of fast neutron alfalfa mutagenesis appropriate radiation dose.