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目的探讨食管癌患者外周血NKG2D+CD8+NKT细胞百分比及血清sMICA含量在免疫逃逸中的作用及临床意义。方法流式细胞仪测定80例患者(其中64例术后患者)及72例健康对照组NKG2D+CD8+NKT细胞百分比;酶联免疫吸附法测定sMICA含量。结果患者NKG2D+CD8+NKT细胞百分比低于对照组(P<0.01);术后低于对照组(P>0.05);术前低于术后(P<0.01);在TNM分期中,Ⅰ、Ⅱ、Ⅲ、Ⅳ期患者依次降低(P<0.01)。患者sMICA明显高于对照组(P<0.01);术后高于对照组(P>0.05);术前高于术后(P<0.01);在TNM分期中,Ⅰ、Ⅱ、Ⅲ、Ⅳ期患者依次增高(P<0.01)。患者sMICA对NKG2D+CD8+NKT细胞有抑制作用(P<0.01)。结论 NKG2D+CD8+NKT细胞及sMICA参与食管癌的免疫逃逸,两者的测定有助于食管癌的免疫治疗、早期诊断、临床分期及判断手术疗效。
Objective To investigate the role of the percentage of NKG2D + CD8 + NKT cells and serum sMICA in immune escape in patients with esophageal cancer and its clinical significance. Methods The percentage of NKG2D + CD8 + NKT cells in 80 patients (including 64 postoperative patients) and 72 healthy controls were measured by flow cytometry. The sMICA content was determined by enzyme-linked immunosorbent assay. Results The percentage of NKG2D + CD8 + NKT cells was lower than that of the control group (P <0.01), lower than that of the control group (P> 0.05), preoperatively lower than that of the control group (P <0.01) Patients in stage Ⅱ, Ⅲ and Ⅳ decreased in turn (P <0.01). The levels of sMICA in patients were significantly higher than those in the control group (P <0.01), higher than those in the control group (P> 0.05), higher than preoperative levels (P <0.01) Patients in turn increased (P <0.01). Patients with sMICA inhibited NKG2D + CD8 + NKT cells (P <0.01). Conclusion Both NKG2D + CD8 + NKT cells and sMICA are involved in the immune escape of esophageal cancer. The determination of NKG2D + CD8 + NKT cells and esophageal cancer may be helpful for the immunotherapy, early diagnosis, clinical staging and curative effect of esophageal cancer.