通常一只鼠脑只能制成一种染色的连续切片,例如火棉胶包埋或石腊包埋的切片都只能进行一种染色,或者只能染髓鞘,或者只能染胞体,不能作两种染色。若用冰冻切片,又常常难以保持连续完整。这对学习和研究鼠脑的形态结构,特别对初学者无疑有不足之处。至于Luxolfast blue与甲酚紫复染法虽可二者兼染,但髓鞘与胞体见于同一切片,于浓密处未免互相掩叠,终觉迷离。为了既便于分别追踪鼠脑的细胞群与纤维束,又便于对比观察细胞和纤维的配布关系,同时也为了重塑与定位的需要,最好能用一只鼠脑制成细胞、髓鞘间隔分片染色的两种连续切片。 Usually a mouse brain can only be made into a serial of stained sections, such as collodion or paraffin embedded sections can only be one kind of staining, or only dyed myelin, or only dysentery, Can not make two kinds of dyeing. If frozen slices, it is often difficult to maintain continuous integrity. This study and study of mouse brain morphology, especially for beginners undoubtedly inadequate. As for the Luxolfast blue and cresyl violet staining method may both dye, but the myelin and the cell body found in the same slice, in the thick place are mutually overlapping, eventually blurred. In order to facilitate the tracing of rat brain cell populations and fiber bundles, respectively, and to facilitate comparison of cell and fiber distribution, as well as the need for remodeling and positioning, it is best to use a mouse brain cells, myelin spacer Slice stained two serial sections.