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目的:组蛋白乙酰化与有丝分裂过程中的多个染色质相关事件有关,但是它在哺乳动物减数分裂过程中的作用仍不清楚。本研究通过观察人卵子体外成熟过程中不同阶段的组蛋白H3K9乙酰化变化模式,以探讨组蛋白乙酰化在减数分裂过程中的作用。方法:我们选择在我院进行单精子显微注射(Intracytoplasmic sperm injection,ICSI)的病人,共收集用于GV期卵子25个,MI期卵子28个用于本研究。将其中一部分直接用4%多聚甲醛固定,另一部分体外培养成熟至MII期,再用4%多聚甲醛固定。采用免疫荧光染色检测不同发育时期卵子的组蛋白H3K9乙酰化状态。结果:免疫荧光染色结果显示,GV期的卵子可检测到明显的H3K9乙酰化,MI期和MII期的卵子的H3K9乙酰化程度逐渐减弱。结论:人类卵子在成熟过程中会发生组蛋白H3K9乙酰化水平的逐渐降低,可能与减数分裂过程中特定的染色体分离、基因表达的重新编程密切相关。
AIM: Histone acetylation is associated with multiple chromatin-related events during mitosis, but its role in mammalian meiosis remains unclear. In this study, histone H3K9 acetylation was observed in different stages of human oocyte maturation to investigate the role of histone acetylation in meiosis. METHODS: We selected 25 patients with GV oocysts and 28 MI patients for our study of Intracytoplasmic sperm injection (ICSI) in our hospital. One part was directly fixed with 4% paraformaldehyde, and the other part was matured to MII stage in vitro, and then fixed with 4% paraformaldehyde. Immunofluorescence staining was used to detect the histone H3K9 acetylation status of eggs at different developmental stages. Results: The results of immunofluorescence staining showed that significant H3K9 acetylation was detected in GV oocytes, and the degree of acetylation of H3K9 in oocytes in MI and MII was gradually decreased. CONCLUSION: The histone H3K9 acetylation level decreases gradually during maturation of human ovum, which may be related to the specific chromosome segregation during meiosis and the reprogramming of gene expression.