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目的 观察脐带血中内皮细胞的前体细胞 ,并研究该前体细胞分化成熟所需条件。方法 采用常规分离方法 ,分离脐带血中的单个核细胞 ,培养于含小牛下丘脑提取液和地塞米松的MCDB131培养液中 ,以获得具有粘附性的细胞 ,于倒置相差显微镜下每日观察两次一并记录。结果初步的实验结果表明脐带血中存在内皮细胞的前体细胞 ,小牛下丘脑提取物对该细胞的生成有促进作用 ,而地塞米松对其生成有抑制作用。结论 初步证实脐带血中有内皮细胞的前体细胞存在 ,并对其分化发育条件有了初步认识 ,为对其进一步的研究及应用打下了基础
Objective To observe the endothelial cell precursor cells in umbilical cord blood and to study the conditions required for the differentiation and maturation of this precursor cell. Methods Mononuclear cells from umbilical cord blood were isolated by routine isolation method and cultured in MCDB131 medium containing calf hypothalamus extract and dexamethasone to obtain adherent cells. The cells were cultured under inverted phase contrast microscope Observed twice recorded. Results The preliminary experimental results showed that endothelial cell precursors existed in umbilical cord blood. The extract of calf hypothalamus promoted the formation of the cells, while dexamethasone inhibited the formation of the cells. Conclusion The preliminary demonstration of the presence of endothelial cells in umbilical cord blood and their preliminary understanding of the differentiation and development conditions lay the foundation for further research and application