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目的:观察地塞米松对哮喘大鼠气道炎症的作用以及对转录因子T-bet、GATA-3表达的影响。方法:36只SPF级SD大鼠随机分为正常对照组(A组)、哮喘模型组(B组)和地塞米松组(C组),每组12只。以卵蛋白(OVA)腹腔注射并雾化吸入制备大鼠哮喘模型,观察3组大鼠气道病理改变;采用ELISA法测定肺泡灌洗液(BALF)中IL-2、IL-4、IL-5和IFN-γ含量;采用RT-PCR和Westem blotting检测T-bet和GATA-3 mRNA和蛋白的表达。结果:①予OVA腹腔注射和雾化,哮喘大鼠模型制备成功:②地塞米松减少Th2型细胞因子(IL-4、IL-5)的表达(P<0.01),对Th1型细胞因子(IL-2,IFN-γ)表达量无明显影响;③地塞米松抑制GATA-3表达(P<0.01),对T-bet表达无明显影响;④相关分析显示B组T-bet蛋白表达量与EOS、L、WA/Pi和ASM/Pi呈负相关(P<0.01);B组GATA-3蛋白表达量与EOS、L、WA/Pi和ASM/Pi呈正相关(P<0.01);B组大鼠T-bet蛋白表达量与GATA-3蛋白表达量呈负相关(P<0.01)。结论:支气管哮喘大鼠存在T-bet低表达,GATA-3高表达,并且与气道炎症关系密切:地塞米松抑制气道炎症,可抑制GATA-3表达,但对T-bet表达无明显影响。
Objective: To observe the effect of dexamethasone on airway inflammation and the expression of T-bet and GATA-3 in asthmatic rats. Methods: Thirty - six SPF SD rats were randomly divided into normal control group (group A), asthma model group (group B) and dexamethasone group (group C) with 12 rats in each group. IL-2, IL-4 and IL-6 in bronchoalveolar lavage fluid (BALF) were measured by ELISA. The asthma model was induced by intraperitoneal injection of nebulized ovalbumin (OVA) 5 and IFN-γ. The mRNA and protein expressions of T-bet and GATA-3 were detected by RT-PCR and Western blotting. (2) Dexamethasone decreased the expression of Th2 cytokines (IL-4, IL-5) (P <0.01), and decreased the expression of Th1 cytokines (P <0.01), and had no significant effect on the expression of T-bet. (4) The correlation analysis showed that the expression of T-bet in group B (P <0.01). The protein expression of GATA-3 in group B was positively correlated with EOS, L, WA / Pi and ASM / Pi There was a negative correlation between T-bet protein expression and GATA-3 protein expression (P <0.01). Conclusion: Bronchial asthma rats have low expression of T-bet and high expression of GATA-3, and are closely related to airway inflammation. Dexamethasone can inhibit airway inflammation, inhibit the expression of GATA-3, but has no obvious effect on T-bet expression influences.