论文部分内容阅读
目的 :研究病毒性肝炎基因芯片的制备 ,用肝炎基因诊断芯片 ,免疫组织化学法和原位分子杂交法 ,检测 99例乙型肝炎后肝硬化肝组织中 HBV DNA,比较各种方法优缺点。方法 :将 PCR扩增的 HBVDNA探针用点样仪点于玻片介质上 ,并经过点样处理后制备成基因芯片 ;收集肝炎后肝硬化肝组织标本 99份 ,分别用基因芯片、原位分子杂交法、免疫组织化学法检测 HBVDNA和 HBc Ag。结果 :5 3例 HBc Ag、HBVDNA阳性组织中基因芯片检测阳性 40例 ;HBc Ag阳性 2 2例中基因芯片检测阳性 6例 ;32例 HBc Ag HBVDNA阴性组织中基因芯片检测均阴性。结论 :肝炎基因诊断芯片可以检测肝组织中 HBVDNA,准确率可达 75 % ,假阳性率低。
OBJECTIVE: To study the preparation of viral hepatitis microarray, detect the HBV DNA in liver cirrhosis caused by hepatitis B by using hepatitis B gene microarray, immunohistochemistry and in situ hybridization. The advantages and disadvantages of various methods were compared. Methods: HBVDNA probes amplified by PCR were spotted on glass slides by spotting, and then prepared into gene chips after spotting treatment. 99 samples of hepatic cirrhosis liver tissues were collected and analyzed by gene chip, Molecular hybridization, immunohistochemical detection of HBVDNA and HBcAg. Results: Gene chip was positive in 40 of HBeAg and HBVDNA positive cases in 5 3 cases, 6 cases of HBcAg positive in 2 cases, and 32 cases of negative HBcAg HBVDNA negative tissue. Conclusion: The hepatitis B gene chip can detect HBVDNA in liver tissue with the accuracy of 75% and the false positive rate low.