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孤啡肽（orphaninFQ，OFQ）是最近发现的一种门肽，为阿片受体家族中一个新成员—“孤儿受体”（orphareceptor）的内源性配基。OFQ在痛觉调制中的作用与内阿片肽明显不同。本实验在大鼠电刺激甩尾测痛模型上，观察了侧脑室（ICV）及鞘内注射（IT）OFQ对伤害性电刺激引起的痛反应及电针镇痛的影响。结果发现，大鼠ICV及IT0．1μg（0.055nmol）OFQ对痛反应无影响，而1.0μg（0.55nmol）的OFQ可明显降低痛阈；但两种剂量的0FQ阴无论是脑室还是鞘内给药，均可对抗电针镇痛；采用反义核苷酸技术阻断OFQ受体合成，大鼠痛阈升高，此时再给予OFQ，则电针镇痛效应无明显改变。表明OFQ对基础痛阈的影响与剂量有关：小剂量无影响，大剂量使痛阈降低。但无论对基础痛阈有无影响，OFQ均对抗电针镇痛。
OrphaninFQ (OFQ) is a recently discovered gate peptide that is a new member of the opioid receptor family, the endogenous ligand of the orphareceptor. The role of OFQ in pain modulation is significantly different from that of endogenous opioid peptides. In this study, we observed the effects of lateral ventricle (ICV) and intrathecal (OFQ) OFQ on nociceptive responses induced by noxious electrical stimulation and electroacupuncture analgesia in rats. The results showed that rat ICV and IT0.1μg (0.055nmol) OFQ had no effect on the pain response, whereas 1.0μg (0.55nmol) OFQ significantly reduced the pain threshold; however, both doses of 0FQ were negative for intraventricular or intrathecal Drugs can be against the electroacupuncture analgesia; use of antisense nucleotide technology to block OFQ receptor synthesis, rat pain threshold increased, and then give OFQ, the electroacupuncture analgesic effect no significant change. The effect of OFQ on basal pain threshold was dose-dependent: small doses had no effect and high doses resulted in lower pain threshold. However, regardless of the basis of pain threshold, OFQ against electroacupuncture analgesia.