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为鉴定全反式维甲酸(ATRA)诱导的人肺癌细胞分化与凋亡相关基因,采用mRNA差异显示的方法对全反式维甲酸处理前后的人肺腺癌细胞系GLC82的mRNA表达模式进行了分析,并获得了一个受ATRA转录上调的cDNA片段RAa7。核酸序列分析表明RAa7是未曾报道过的新序列。Northern杂交结果显示RAa7可能是一个组织特异性表达的基因,其主要转录本约1.4kb。在全反式维甲酸和N-(4-羟苯)维甲酰胺(4HPR)诱导的其他几种肺癌细胞的分化与凋亡过程中RAa7基因呈降调节。该基因的升或降调节可能都参与了细胞分化或凋亡的过程。
In order to identify all-trans retinoic acid (ATRA)-induced genes involved in differentiation and apoptosis of human lung cancer cells, mRNA expression patterns were used to analyze mRNA expression pattern of human lung adenocarcinoma cell line GLC82 before and after treatment with all-trans retinoic acid. Analysis and acquisition of a cDNA fragment RAa7 up regulated by ATRA transcription. Nucleic acid sequence analysis showed that RAa7 is a novel sequence that has not been reported. Northern blot results showed that RAa7 may be a tissue-specific gene, with its major transcript of about 1.4 kb. In the process of differentiation and apoptosis of other lung cancer cells induced by all-trans retinoic acid and N-(4-hydroxyphenyl) retinamide (4HPR), RAa7 gene was down-regulated. The up- or down-regulation of this gene may be involved in the process of cell differentiation or apoptosis.