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神经生长因子的研究是目前医学生物学研究的热点之一,其诱导瘤细胞的分化更引人注目。我们的研究发现,神经生长因子仅能诱导具有短神经突起的N型瘤细胞分化成神经元样的细胞。这些细胞均无N-myc扩增,均具有高、低亲和性神经生长因子受体。而对神经生长因子无反应的细胞系或为S型细胞,或缺少神经生长因子受体的表达,或有N-myc扩增。进一步研究发现,不同细胞类型代表着瘤细胞的不同分化方向。神经生长因子仅能诱导向神经元方向分化的细胞分化。分化的瘤细胞中,C-myc的表达明显下降,提示神经生长因子可能通过抑制C-myc的表达而促进分化。用基因重组技术,恢复瘤细胞中神经生长因子受体的表达,可部分恢复其对神经生长因子的反应。针对N-myc的反义基因调控,可促进其分化过程。对瘤细胞原位凋亡检测和细胞凋亡相关基因的研究表明:诱导分化过程中伴随着bcl-2表达的下降和瘤细胞凋亡的增加。
Nerve growth factor research is currently one of the hot spots in the study of medical biology, which induces the differentiation of tumor cells more striking. Our study found that NGF can only induce N-type tumor cells with short neurites to differentiate into neuron-like cells. None of these cells have N-myc amplification, both with high and low affinity nerve growth factor receptors. Whereas non-responsive cell lines or S-type cells, or lacking nerve growth factor receptor expression, or N-myc amplification. Further study found that different cell types represent different differentiation directions of tumor cells. Nerve growth factor can only induce cell differentiation towards neuronal differentiation. Differentiated tumor cells, C-myc expression was significantly decreased, suggesting that nerve growth factor may inhibit the expression of C-myc and promote differentiation. Using gene recombination technology to restore the expression of nerve growth factor receptor in tumor cells can partially restore its response to nerve growth factor. Antisense gene regulation of N-myc can promote its differentiation process. The in situ apoptosis of tumor cells and apoptosis-related genes studies have shown that: during the induction of differentiation accompanied by a decrease in bcl-2 expression and tumor cell apoptosis increased.