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目的:探讨Survivin、PTEN、p16基因在大肠癌中的表达及其临床意义。方法:利用免疫组织化学SP法检测43例结直肠癌和12例正常结直肠粘膜组织中以上3种基因的表达产物。结果:43例结直肠癌组织中Survivin基因表达23例,癌旁正常直结肠组织中无Survivin基因的表达,Survivin的表达与结直肠癌的组织分型、浸润深度、Dukes’分期无明显相关性(P＞0．05),与结肠癌的转移有相关性(P＜0．05)。而PTEN,p16与大肠癌的分化程度、浸润深度、临床分期、淋巴结转移有关(P＜0．05)。结论:Survivin、PTEN、p16的异常表达在大肠癌发生、发展过程中起重要作用,联合检测Survivin、PTEN、p16表达水平有可能作为反映大肠癌进展和预后的生物学指标。
Objective: To investigate the expression of Survivin, PTEN and p16 in colorectal carcinoma and its clinical significance. Methods: Immunohistochemical SP method was used to detect the expression products of the above three genes in 43 cases of colorectal cancer and 12 cases of normal colorectal mucosa. Results: There was no expression of Survivin gene in 43 cases of colorectal cancer tissues and no expression of Survivin in adjacent normal colorectal tissues. The expression of Survivin had no significant correlation with the histological type, depth of invasion and Dukes’ stage of colorectal cancer (P> 0.05), which was correlated with the metastasis of colon cancer (P <0.05). However, PTEN and p16 were correlated with the degree of differentiation, depth of invasion, clinical stage and lymph node metastasis (P <0.05). CONCLUSIONS: The abnormal expression of Survivin, PTEN and p16 plays an important role in the carcinogenesis and progression of colorectal cancer. Combined detection of Survivin, PTEN and p16 expression may be used as biological indicators to reflect the progress and prognosis of colorectal cancer.