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采用反相高效液相色谱法，以咖啡因为内标，测定了氢溴酸高乌甲素片含量。ＲＰ－１８ＯＤＳ５ｕｍ柱，甲醇－磷酸二氢钠（７２：２８Ｖ／Ｖ）为流动相，ＵＶ２５２ｎｍ波长处检测。在０．０４～０．２０ｍｇ／ｍｌ范围内，峰面积与浓度呈良好的线性关系。氢溴酸高乌甲素片的平均回收率为１００．２％，相对标准偏差为１．２％。本法快速、简便、灵敏。
The content of lappaconitine hydrobromide was determined by RP-HPLC with caffeine as internal standard. RP-18ODS5um column, methanol-sodium dihydrogen phosphate (72: 28V / V) as the mobile phase, UV252nm wavelength detection. In the range of 0.04 ~ 0.20mg / ml, the peak area and concentration showed a good linear relationship. The average recovery of lappaconitine hydrobromide tablets was 100.2% with a relative standard deviation of 1.2%. This law is quick, easy and sensitive.