论文部分内容阅读
背景:国内外对血管紧张素I转换酶基因插入/缺失多态性与人体运动能力关联性的研究较多。但是,实验对象(如种族差异)、方法、条件控制不同,所得的结论并不一致。人体800m跑运动能力的高低能较好地反映人体的速度耐力素质。目的:探讨人体血管紧张素I转换酶基因插入/缺失多态性与速度耐力之间的关联性。设计:对照观察。单位:解放军体育进修学院科研所体能中心。对象:于2004-06/07选择解放军体育学院(现改为解放军体育进修学院)健康汉族男性40名,年龄(21±2)岁,运动史基本相同,无心血管疾病和糖尿病史。方法:抽取受试者外周静脉血3mL,采用聚合酶链反应扩增技术检测受试者基因型;采用YSI1500型乳酸分析仪测定安静状态、800m跑后血乳酸浓度,记录800m跑成绩。并对成绩进行逐步聚类分析,将受试者分为高速度耐力组和低速度耐力组。主要观察指标:血管紧张素I转换酶基因型测定、800m跑成绩测定、血乳酸浓度测定。结果:纳入受试对象40名,均进入结果分析。①血管紧张素I转换酶基因型有3种:II型(490bp)、DD型(190bp)、ID型(190,490bp)。②将800m跑成绩按升序分为3类:分别为前18名,中间21名,最后1名。方差分析结果显示,类别间距离差异的概率均为0.000,聚类效果较好,将第一类归为高速度耐力组18名,第二、三类归为低速度耐力组22名。③高速度耐力组和低速度耐力组组间血管紧张素I转换酶基因型分布及等位基因分布差异均无显著性意义(P>0.05)。④3种基因型组人群800m跑后血乳酸浓度较安静状态时显著升高,差异有显著性意义[II型基因分别为(14.93±2.46),(2.17±0.56)mmol/L;ID型基因分别为(14.86±1.54),(2.41±0.70)mmol/L;DD型基因分别为(14.13±1.51),(1.99±0.27)mmol/L,P<0.01];但无论是安静状态、800m跑后,还是800m跑后与安静状态差值,各基因型组间差异均无显著性意义(P>0.05)。结论:人体血管紧张素I转换酶基因插入/缺失多态性与速度耐力无关。
Background: There are many researches on the association between the insertion / deletion polymorphism of angiotensin I converting enzyme gene and the exercise capacity of human body at home and abroad. However, the experimental subjects (such as racial differences), methods and conditions of control are different, the conclusions obtained are not consistent. The level of 800m body movement ability of the human body can better reflect the speed of the endurance quality. Objective: To investigate the association of human angiotensin I converting enzyme gene insertion / deletion polymorphism with speed and endurance. Design: Controlled observation. Unit: People’s Liberation Army Institute of Physical Education Institute physical fitness center. Target: Selected from PLA Sports Academy (now renamed PLA Sports Academy) 2004-06 / 07 40 healthy Han nationality men aged 21 ± 2 years old with the same exercise history and no history of cardiovascular disease and diabetes. Methods: 3mL peripheral venous blood was drawn from the subjects and genotypes were detected by polymerase chain reaction amplification. The resting state was measured by YSI1500 lactic acid analyzer, and the blood lactic acid concentration after 800m was recorded. Gradual clustering analysis was performed on the scores, and the subjects were divided into high-speed endurance group and low-speed endurance group. MAIN OUTCOME MEASURES: Determination of angiotensin I converting enzyme genotype, 800m running score, blood lactate concentration. Results: 40 subjects were enrolled in the result analysis. ① angiotensin I converting enzyme genotypes have three types: type II (490bp), DD type (190bp), ID type (190,490bp). ② 800m running results will be divided into three categories in ascending order: the first 18, the middle 21, the last one. Analysis of variance showed that the probability of the difference between the categories was 0.000, the clustering effect was better, the first category was classified as high speed endurance group 18, the second and third categories were divided into low speed endurance group 22. ③ There was no significant difference in genotype distribution and allele distribution of angiotensin I converting enzyme between high-speed endurance group and low-speed endurance group (P> 0.05). ④ The blood lactate concentrations in the three genotype groups after 800m run were significantly higher than those in the rest state [14.93 ± 2.46 and 2.17 ± 0.56 mmol / L, respectively]; ID genotypes were Were (14.86 ± 1.54) and (2.41 ± 0.70) mmol / L, respectively. DD genotypes were (14.13 ± 1.51) and (1.99 ± 0.27) mmol / L, , Or 800m after the run and the difference between the quiet state, no significant difference between the genotypes (P> 0.05). Conclusion: The insertion / deletion polymorphism of human angiotensin I converting enzyme gene has nothing to do with speed and endurance.