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本文检测了96例各类肾小球疾病患者血清抗肾小球基底膜(CRM)抗体。ELISA显示19例患者抗GBM抗体用性,占19.7％,其中5例肾组织免疫荧光显示免疫球蛋白沿肾小球基底膜呈颗粒性沉积者中有3例抗GBM抗体阳性,提示部分GBM颗粒性沉积者可能与抗GBM抗体的参与有关。进一步应用免疫印迹法显示7例患者分别存在抗层连蛋白(Laminin)、抗硫酸肝素蛋白多糖(HSPG)、扰Ⅳ受胶原7S抗体。本组病例中无一例临床诊断Goodpasture综合征,也无一例存在抗Ⅳ型胶原NC_1抗体,因而也反证了抗Ⅳ型胶原NC_1抗体对Goodpesture综合征的特异性诊断意义。
This article tested 96 cases of various glomerular diseases in patients with anti-glomerular basement membrane (CRM) antibody. Nineteen patients had anti-GBM antibodies, accounting for 19.7% of them, of which 5 showed anti-GBM antibodies positive by immunofluorescence staining of immunoglobulin along the glomerular basement membrane, indicating that some GBM particles Sexual depositors may be involved in the anti-GBM antibody. Further immunoblotting showed that there were 7 patients with anti-laminin (Laminin), anti-heparin sulfate proteoglycan (HSPG) and interferon Ⅳ by collagen 7S antibody respectively. No case of this group of patients with clinical diagnosis of Goodpasture syndrome, and no case of anti-type Ⅳ collagen NC_1 antibody, which also contradicts the anti-type Ⅳ collagen NC_1 antibody Good Good for the diagnosis of Goodpesture syndrome significance.