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目的 调查中国武汉地区汉族人群STR基因座—D3S1 358、D1 3S31 7、D1 2S391基因频率分布和群体遗传数据。方法 从 2 0 8个汉族无关个体收集血液标本 ,应用PCR技术及聚丙烯酰胺凝胶垂直板电泳对D3S1 358、D1 3S31 7和D1 2S391基因座分型。结果 D3S1 358检出 7个等位基因和 4 1个基因型。三基因座基因型分布符合Hardy-Weinberg平衡。观察 2 31次减数分裂均未发现突变基因。另外 ,调查结果计算显示D3S1 358、D1 3S31 7和D1 2S391基因座的杂合度 (H)分别为 0 70 98、 0 80 56和 0 84 0 0 ;三个人识别能力 (DP)分别为 0 851 6、 0 9332和 0 952 3;非父排除率 ( pE)分别为 0 4 463、 0 60 1 6和 0 681 8。结论 D3S1 358、D1 3S31 7和D1 2S391基因座在群体遗传学研究和法医学亲子鉴定及个人识别中具有较高实用价值
Objective To investigate the frequency distribution and population genetic data of STR loci-D3S1 358, D1 3S31 7, D1 2S391 in Han nationality in Wuhan, China. Methods Blood samples were collected from 208 unrelated Han individuals. The D3S1 358, D1 3S31 7 and D1 2S391 loci were genotyped by PCR and polyacrylamide gel electrophoresis. Results D3S1 358 detected 7 alleles and 4 1 genotypes. The genotypes of the three loci are in accordance with the Hardy-Weinberg equilibrium. No mutations were found in 231 meiosis cases. In addition, the results of the survey showed that the heterozygosities (H) of D3S1 358, D1 3S31 7 and D1 2S391 loci were 0 70 98, 0 80 56 and 0 84 0 0, respectively. The three individuals’ recognition ability (DP) was 0 851 6 , 0 9332 and 0 952 3 respectively; and the non-parent exclusion rates (pE) were 0 4 463, 0 60 1 6 and 0 681 8, respectively. Conclusion The D3S1 358, D1 3S31 7 and D1 2S391 loci have high practical value in population genetics and forensic paternity testing and personal identification