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【目的】观察苦参碱对K-ras基因突变型结肠癌SW480细胞增殖的抑制作用及机制。【方法】以不同浓度苦参碱干预K-ras基因突变型人结肠癌SW480细胞,采用新型四唑氮盐(MTS)做比色分析测定细胞增殖,流式细胞技术(FCM)检测细胞凋亡,细胞划痕法观察细胞侵袭转移能力的变化,Western blotting方法检测细胞内丝裂原活化蛋白激酶(MAPK)通路下游关键激酶MEK1/2蛋白的表达。【结果】与空白对照组比较,0.125~1 mg/m L浓度的苦参碱能显著抑制SW480细胞增殖,促进细胞凋亡,有效抑制结肠癌细胞的移行,降低MEK1/2蛋白的表达(P<0.05),其作用强度随药物浓度增加有升高的趋势。【结论】苦参碱可能通过下调MAPK信号通路下游MEK1/2蛋白的表达,有效抑制SW480细胞的增殖和移行,并促进其凋亡。
【Objective】 To observe the inhibitory effect and mechanism of matrine on the proliferation of K-ras mutant human colon cancer SW480 cells. 【Methods】 Matrine (K-ras) mutant human colon cancer SW480 cells were treated with different concentrations of matrine. Cell proliferation was measured by colorimetric assay using novel tetrazolium salt (MTS), and apoptosis was detected by flow cytometry (FCM) The cell invasion and metastasis were observed by cell scratch assay. The expression of MEK1 / 2, a key kinase downstream of mitogen-activated protein kinase (MAPK) pathway, was detected by Western blotting. 【Results】 Compared with the blank control group, matrine at concentration of 0.125 ~ 1 mg / m L could significantly inhibit the proliferation of SW480 cells and promote apoptosis, effectively inhibit the migration of colon cancer cells and decrease the expression of MEK1 / 2 protein (P <0.05). The intensity of action increased with the increase of drug concentration. 【Conclusion】 Matrine may inhibit the proliferation and migration of SW480 cells and promote its apoptosis through down-regulating the expression of MEK1 / 2 protein downstream of MAPK signaling pathway.