报道了一种新的黄瓜黑星病抗病性鉴定方法——离体子叶接种法,并对接种鉴定条件进行了研究。该方法将刚展平的子叶剪下铺到有二层滤纸的培养皿内保湿,(?)滴管将10~4～10~5个·mL~(-1)孢子浓度的悬浮液,点滴接种到离体子叶的上表皮,每滴约0.02 mL.在15～20℃,黑暗条件下鉴定,接种后5 d 调查病情。这一方法简单、可靠、不影响幼苗继续生长。 A new method for the identification of disease resistance in cucumber scab was reported, in vitro cotyledon inoculation method, and the conditions of inoculation were studied. In the method, the freshly-cotyledon cotyledons are cut out and moistened in a petri dish having a two-layer filter paper, and the suspension of (10 ~ 4 ~ 10 ~ 5 · mL ~ (-1) Inoculated into the excised cotyledons on the epidermis, each drop of about 0.02 mL at 15 ~ 20 ℃, dark conditions identified 5 days after inoculation investigation of the disease. This method is simple and reliable, does not affect the seedlings continue to grow.