目的 探讨蚊虫抗药性敏感测试方法,方法 采用微板法,对室内常规饲养的敏感品系和室内选育的DDVP、抗残杀威品系淡色库蚊非特异性酯酶的活性进行了测定。结果 敏感品系蚊虫OD值≥0.9的占17.16％(35/204条);<0.9的占82.84％(169/204条);抗DDVP品系OD值≥0.9的占83.33％(200/240条);<0.9的占16.67％(40/240条);抗残杀威品系≥0.9的占74.89％(176/235条),<0.9的占25.11％(59/235条)。结论 敏感品系与抗性品系淡色库蚊的平均OD值存在较大差异。 Objective To explore the sensitive test method of mosquito resistance, and to determine the non-specific esterase activity of Culex pipiens pallens DDVP and anti-malarial strains susceptible to conventional indoor feeding by microplate method. Results The susceptible strain mosquitoes accounted for 17.16% (35/204) with OD ≥0.9, 82.84% (169/204) with <0.9, and 83.33% (200/240) with OD value≥0.9 of DDVP strains. <0.9 accounted for 16.67% (40/240); anti-malaria strains ≥0.9 accounted for 74.89% (176/235), <0.9 accounted for 25.11% (59/235). Conclusion The average OD values ​​of Culex pipiens pallens between susceptible and resistant strains are quite different.