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目的 :观察转化生长因子β1 (TGF-β1 )及其受体 I(TβR1 )基因在人类胃癌组织中的表达及其与胃癌病理生物学行为的关系。方法 :应用抗 TGF-β1 和 TβR1 多克隆抗体对 10 7例胃癌组织中的 TGF-β1 和 97例胃癌组织中的 TβR1 基因表达蛋白进行了 S- P免疫组织化学方法检测。结果 :TGF-β1 在胃癌组织中的阳性表达 (75 % )明显高于癌旁组织 (2 4.4% )及正常组织 (2 9.2 % ) ,差异非常显著 (P <0 .0 1) ;TRβ1 在胃癌组织中阳性表达 (19.6 % )明显低于癌旁组织 (79.6 % )和正常组织 (75 .6 % ) ,差异有显著性 (P <0 .0 5 )。结论 :TGF-β1 和 TβR1 基因的异常表达与胃癌的发展及恶性程度有关。
OBJECTIVE: To observe the expression of transforming growth factor β1 (TGF-β1) and its receptor I (TβR1) gene in human gastric carcinoma and its relationship with the pathological and biological behavior of gastric cancer. Methods: Polyclonal anti-TGF-β1 and TβR1 antibodies were used to detect the expression of TβR1 gene in 10 7 gastric cancer tissues and 97 gastric cancer tissues by S-P immunohistochemistry. Results: The positive expression of TGF-β1 in gastric cancer tissue was significantly higher than that in paracancer tissue (2 4.4%) and normal tissue (2 9.2%), the difference was significant (P0.01) The positive expression in gastric cancer tissues (19.6%) was significantly lower than that in paracancerous tissues (79.6%) and normal tissues (75.6%), the difference was significant (P <0.05). Conclusion: The abnormal expression of TGF-β1 and TβR1 genes is related to the development and malignancy of gastric cancer.