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应用多聚酶链反应技术(PCR)体外扩增脑脊液中结核分枝杆菌DNA,酶斑免疫结合技术检测结核杆菌蛋白抗原及ABC-ELISA法检测相应抗体联合检测诊断结核性脑膜炎,阳性率分别为:83.7%(36/43),76.7%(33/43),74.4%(32/43)。讨论三者间发病不同期的关系,三者有阳性交叉现象。
Mycobacterium tuberculosis DNA in cerebrospinal fluid was amplified by polymerase chain reaction (PCR) in vitro. Mycobacterium tuberculosis antigen was detected by enzyme-linked immunosorbent assay (ABC-ELISA) and combined detection of corresponding antibodies by ABC-ELISA. The positive rates were: 83.7% (36/43), 76.7% (33/43), 74.4% (32/43). Discuss the relationship between the three different stages of the disease, the three positive cross phenomenon.