一、MAP与跨膜动作电位的关系吸引电极方法最早是在1936年由Shutz首创的。其后在1949年被玻璃微电极技术所取代,但从1964年开始,由于该法便于在动物和人身上做在体MAP记录,又重新引起人们的重视。玻璃微电极细胞内记录方法,技术条件比较复杂,目前尚难用于较大动物在体心脏做较长时间的观察,因为较大动物的心脏跳动十分猛烈,而强行限制跳动的方法,又会影响正常的心功能和电活动。但利用吸引电极的方法能够十分简便地在在位心脏上记录出与跨膜动作电位波形相似的单相动作电位(monophasic action potential,简写为MAP)。根据不少学者的研究,一般都认为吸引电极记录的MAP是反映跨膜动作电位复极过程的时程和波形变化的可信指标。Hoffman等一些学者曾比较了在同一个心脏上同时用吸引电极和玻璃微电极记 First, the relationship between MAP and transmembrane action potential The method of attracting electrodes was first pioneered by Shutz in 1936. It was later replaced by glass microelectrode technology in 1949, but since 1964 it has gained renewed attention because of its ease of recording in vivo MAPs in animals and humans. Glass microelectrode intracellular recording method, the technical conditions are complex, is currently difficult to use for larger animals in the body to do a longer observation of the heart, because larger animals beat the heart very violent, and forced to limit the beating method, will Affect normal heart function and electrical activity. However, the monophasic action potential (MAP) similar to the transmembrane action potential waveform can be very easily recorded on the eutopic heart using the method of attracting electrodes. According to many scholars’ studies, it is generally believed that the MAP that attracts electrode recording is a reliable indicator of the time course and waveform change of the repolarization process of transmembrane action potentials. Some scholars such as Hoffman have compared the same heart with a suction electrode and a glass microelectrode