一、接种源的调整 1.使用菌株:甜瓜蔓枯病菌极易分化,虽然认为在田间分布着很多致病力不同的菌株,但是迄今的鉴定结果,没有发现抗病性逆转的现象。然而,使用致病力强的菌株,可以使抗病性鉴定趋于稳定。 2.孢子的增殖:1973年由岸氏发明孢子增殖法后,鉴定技术开始迅速地发展起来。其方法是:首先制做含少量琼胶(琼胶量5g/e的马铃薯煎汁培养基,将其培养基倒入培养皿中,然后接种蔓枯病病原菌孢子或菌丝于其内,随之并将其置于22—24℃的室 First, the adjustment of the source of vaccination 1. The use of strains: melon powdery mildew easily differentiated, although considered in the field distribution of many strains of different virulence, but so far the identification results did not find the phenomenon of disease resistance reversal. However, the use of virulent strains allows the identification of disease resistance to stabilize. 2. Spore proliferation: In 1973 invented by the Shore spore proliferation method, the identification of technology began to rapidly develop. The method is as follows: First, a small amount of agar (the amount of agar 5g / e potato dextrose medium, the medium into the petri dish, and then inoculated with the gummy pathogen spores or mycelium, with And placed in a chamber at 22-24 ° C