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艰难梭菌是一种芽胞杆菌,可产生毒素介导性疾病。艰难梭菌表达2种主要毒力因子,即毒素A(TcdA)和毒素B(TcdB)。在人类和动物研究中结果表明,人类抗这些毒素的免疫力与抗艰难梭菌感染(CDI)明确相关,已知TcdA和TcdB的受体结合域(RBDs)是具免疫原性的。在本项研究中,我们检测了鼠伤寒沙门菌鞭毛蛋白(FliC)亚单位D1作为先天免疫激动剂,在小鼠模型中作为重组融合疫苗表达的靶向TcdA和TcdB的RBDs的免疫佐剂特性。经腹腔免疫的小鼠在血清中出现了明显的抗TcdA和抗TcdB的IgG。在能够密切体现人类疾病的CD1小鼠模型中进行了重组疫苗(加或不加佐
Clostridium difficile is a Bacillus that produces toxin-mediated diseases. C. difficile expresses two major virulence factors, TcA and TcdB. Results from human and animal studies indicate that the human immunity against these toxins is clearly linked to anti-Clostridium difficile infection (CDI), and that the receptor-binding domains (TDRs) of TcdA and TcdB are known to be immunogenic. In this study, we tested the immunoadjuvant properties of Salmonella typhimurium flagellin (FliC) subunit D1 as an innate immune agonist and RBDs targeting TcdA and TcdB expressed as a recombinant fusion vaccine in a mouse model . Peritoneal immunized mice developed significant anti-TcdA and anti-TcdB IgG in the serum. Recombinant vaccines were performed in a CD1 mouse model that closely mirrors human disease (plus or minus