Objective: To examine the expressions of osteopontin (OPN), αVβ3 and Pim‐1 in non‐small cell lung cancer (NSCLC), and investigate their potential pathogenic roles in the development of NSCLC. Methods: Immunohistochemistry was used to examine the expressions of OPN, αVβ3 and Pim‐1 in cohort (136 cases) of NSCLC samples and their adjacent normal lung tissue specimens. Statistical analysis was performed to evaluate the relationships among expressions of OPN, αVβ3 and Pim‐1 and their associations with patients clinico‐pathological parameters. Results: The expressions of OPN and Pim‐1 were predominantly observed in cytoplasm. The expression of αVβ 3 was mostly detected in cytoplasm and/or membrane. In NSCLC samples, the positive rates of OPN, αVβ3 and Pim‐1 expressions were 68.4% (93/136), 77.2% (105/136) and 57.4% (78/136), respectively. In normal lung tissues, in contrast, the positive rates of OPN, αVβ3 and Pim‐1 were 24.0% (12/50), 26.0% (13/50) and 16.0% (8/50), respectively. There were significant differences of the positive expression rates of OPN, αVβ3 and Pim‐1 between NSCLCs samples and normal lung tissues (P<0.01). In addition, the positive expression of OPN, α Vβ3 and Pim‐1 in NSCLCs samples was significantly associated with increased pathological grade, lymph node metastasis and advanced clinical stage (P<0.01), and they were independent of other clinicopathological parameters (P>0.05). Furthermore, a significantly positive correlation between the expression of OPN and αVβ3 (r=0.38, P<0.01), OPN and Pim‐1 (r=0.37, P<0.01), or αVβ3 and Pim‐1 (r=0.20, P<0.05) was evaluated in our NSCLC cohort. Conclusion: OPN, αVβ3 and Pim‐1 proteins are frequently overexpressed in NSCLC, and they may play important roles in the development and/or progression of NSCLC. Objective: To examine the expression of osteopontin (OPN), αVβ3 and Pim-1 in non-small cell lung cancer (NSCLC), and investigate their potential pathogenic roles in the development of NSCLC. Methods: Immunohistochemistry was used to examine the expressions of OPN, αVβ3 and Pim-1 in cohort (136 cases) of NSCLC samples and their adjacent normal lung tissue specimens. Statistical analysis was performed to evaluate the relationships among expressions of OPN, αVβ3 and Pim‐1 and their associations with patients clinico‐pathological Parameters. Results: The expressions of OPN and Pim‐1 were predominantly observed in cytoplasm. The expression of αVβ 3 was mostly detected in cytoplasm and/or membrane. In NSCLC samples, the positive rates of OPN, αVβ3 and Pim‐1 expressions were 68.4% (93/136), 77.2% (105/136) and 57.4% (78/136), respectively. In normal lung tissues, in contrast, the positive rates of OPN, αVβ3 and Pim‐1 were 24.0% (12 /50), 26.0% (13/50) and 16.0% (8/ 50), respectively. There were significant differences of the positive expression rates of OPN, αVβ3 and Pim-1 between NSCLCs samples and normal lung tissues (P<0.01). In addition, the positive expression of OPN, α Vβ3 and Pim‐1 In NSCLCs samples was significantly associated with increased pathological grade, lymph node metastasis and advanced clinical stage (P<0.01), and they were independent of other clinicopathological parameters (P>0.05). αVβ3 (r=0.38, P<0.01), OPN and Pim‐1 (r=0.37, P<0.01), or αVβ3 and Pim‐1 (r=0.20, P<0.05) was evaluated in our NSCLC cohort. OPN, αVβ3 and Pim-1 proteins are frequently overexpressed in NSCLC, and they may play important roles in the development and/or progression of NSCLC.