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应用透射电镜技术对外施水杨酸(salicylic acid,SA)的杏花在低温胁迫处理后花瓣、花粉、柱头和胚珠珠心细胞的超微结构进行了观察。结果表明,喷水对照经–2℃低温处理4 h后,花瓣细胞结构受到了严重破坏,发生了质壁分离,线粒体内嵴结构被破坏;花粉壁外壁结构破坏明显;柱头细胞和胚珠珠心细胞的线粒体内嵴发生囊泡化甚至解体呈空洞状,细胞核双层核膜发生溶解或部分消失。而外施100μmol·L-1 SA的杏花花瓣、花粉、柱头和珠心细胞在–2℃低温处理4 h后,依然保持与正常细胞相似的细胞器结构。这说明SA预处理可增强在低温下细胞结构的稳定性,从而提高杏花的低温适应性,从细胞学角度证实了外施适宜浓度的SA可增强杏花的抗寒性。
The ultrastructure of apricot flower of salicylic acid (SA) exposed to low temperature stress was observed by transmission electron microscopy (TEM). The ultrastructure of petal, pollen, stigma and ovules were observed. The results showed that the structure of petal cells was severely damaged, the wall of mitochondria was damaged, the structure of mitochondria cristae was destroyed, the structure of pollen wall was destroyed obviously, and the cell wall of stigma cells and ovules The mitochondrial inner crest cells were vacuolized and even disintegrated, and the nuclear bilayer nuclear membrane dissolved or partially disappeared. However, the apricot flower petal, pollen, stigma and pearl heart cells exposed to 100μmol·L-1 SA still maintain the organelle structure similar to that of normal cells after being treated for 4 h at -2 ℃. This shows that SA pretreatment can enhance the stability of cell structure at low temperature, thereby improving the low temperature adaptability of apricot, cytologically confirmed the appropriate application of appropriate concentrations of SA can enhance the apricot cold resistance.