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为了研究结核分枝杆菌国际标准强毒株H37Rv株和卡介苗菌株(BCG)分别感染巨噬细胞RAW264.7细胞株对细胞内转铁蛋白受体和铁蛋白表达量的时相变化及意义,分别于感染后1,6,12,18,24 h,应用ELISA和Western blot检测各组巨噬细胞中TfR和Fn的表达量。ELISA检测结果显示,各组感染巨噬细胞上清液中TfR表达量均高于对照组,在感染12,18 h差异最明显,具有统计学意义(P<0.05)。Western blot检测结果显示:于模型建成后1,6,18 h各感染组差异有统计学意义(P<0.05)。用ELISA和Western blot检测显示各组小鼠巨噬细胞内Fn随时间变化表达逐渐增强,在1 h时Fn的表达为对照组高于H37Rv组和BCG组;6 h后,感染组表达逐渐高于对照组,12 h时,H37Rv组>对照组,差异具有统计学意义(P<0.05),在18 h和24 h时,H37Rv组>BCG组>对照组,差异具有统计学意义(P<0.05)。结核分枝杆菌感染导致巨噬细胞内TfR和Fn表达量增高,且与菌株毒力强弱有关,毒力强的菌株感染巨噬细胞的TfR和Fn的表达量更高。
In order to study the time-dependent changes of intracellular transferrin receptor and ferritin in macrophage RAW264.7 cells infected with Mycobacterium tuberculosis H37Rv strain and BCG strain respectively, At 1, 6, 12, 18 and 24 h after infection, the expression of TfR and Fn in each group of macrophages were detected by ELISA and Western blot. The results of ELISA showed that the expression of TfR in macrophage supernatant of each group was higher than that of the control group, and the difference was significant at 12 and 18 h (P <0.05). Western blot results showed that there was significant difference between the infected groups at 1, 6, 18 h after model establishment (P <0.05). The results of ELISA and Western blot showed that the expression of Fn in macrophages increased gradually with time and the expression of Fn in control group was higher than that in H37Rv group and BCG group at 1 h. At 12 h, H37Rv group> control group, the difference was statistically significant (P <0.05). At 18 h and 24 h, H37Rv group> BCG group> control group, the difference was statistically significant (P < 0.05). Mycobacterium tuberculosis infection resulted in increased expression of TfR and Fn in macrophages, and was related to the virulence of strains. TfR and Fn expression of macrophages infected by virulent strains were higher.