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目的建立超高效液相色谱质谱联用法(UPLC-MS/MS)同时测定儿童血浆中利福平、利福喷丁和利福布汀的浓度方法。方法以扎来普隆为内标,血浆样品经乙腈沉淀蛋白处理后检测。ACQUITY UPLC HSS T3色谱柱(2.1 mm×100 mm,1.8μm)为分析柱,以乙腈-水(15 mmol·L-1甲酸铵-0.05%甲酸)为流动相,梯度洗脱,用电喷雾离子化源(ESI),以正离子多反应监测(MRM),检测专属性、标准曲线与定量下限、精密度与回收率、基质效应和稳定性。结果利福平、利福喷丁和利福布汀的线性范围分别为100~5000,20~2000,20~2000 ng·m L-1。日内、日间精密度相对标准偏差(RSD)均小于15%。结论本法灵敏、简便、准确,可同时对利福平、利福喷丁及利福布汀进行治疗药物监测。
Objective To establish a method for the simultaneous determination of rifampicin, rifapentine and rifabutin in children by UPLC-MS / MS. Methods Zaleplon was used as an internal standard, and plasma samples were detected after treatment with acetonitrile precipitated protein. The ACQUITY UPLC HSS T3 column (2.1 mm × 100 mm, 1.8 μm) was used as the analytical column. The mobile phase consisted of acetonitrile-water (15 mmol·L-1 ammonium formate-0.05% formic acid) ESI, with positive ion multiple reaction monitoring (MRM), assay specificity, standard curve and quantitation limits, precision and recovery, matrix effects and stability. Results The linear ranges of rifampin, rifapentine and rifabutin were 100-5000, 20-2000 and 20-2000 ng · m L-1, respectively. The relative standard deviations (RSDs) of intra-day and inter-day precision were less than 15%. Conclusion This method is sensitive, simple and accurate, and can simultaneously monitor the therapeutic drugs for rifampin, rifapentine and rifabutin.