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目的:通过检测PDCD5及抗核抗体谱在自身免疫性疾病中的表达。方法:应用酶联免疫吸附法(ELISA法)对117例自身免疫病患者及32例正常人外周血PDCD5表达和抗核抗体谱检测,观察二者在自身免疫性疾病中的相关性。结果:117例自身免疫性疾病患者抗核多肽抗体谱中,抗核抗体有不同程度的变化,SSA阳性率最高,总阳性率为76.9%。在诊断为SLE的患者中SSA、SSA(Ro52)、dsDNA的阳性率较高,分别为81%、51%、51%。在SS患者中SSA、SSB的阳性率较高分别为88%、75%,健康对照组未检出抗核抗体。自身免疫患者与健康对照组外周血PDCD5含量比较显示对照组与SLE、RA、ITP、SS患者的PDCD5含量存在显著性差异(P<0.05),且在SLE患者中PDCD5含量最高。结论:自身免疫病患者PDCD5存在着异常表达,抗核抗体谱检测对自身免疫病有一定的提示作用。
Objective: To detect the expression of PDCD5 and anti-nuclear antibody in autoimmune diseases. Methods: The expression of PDCD5 in peripheral blood of 117 patients with autoimmune diseases and 32 healthy controls were detected by enzyme - linked immunosorbent assay (ELISA). The relationship between them in autoimmune diseases was observed. Results: In the anti-nuclear peptide antibody spectrum of 117 patients with autoimmune diseases, anti-nuclear antibodies varied in varying degrees. The positive rate of SSA was the highest, with a total positive rate of 76.9%. The positive rates of SSA, SSA (Ro52) and dsDNA were 81%, 51% and 51% in patients diagnosed as SLE, respectively. In SS patients, the positive rates of SSA and SSB were 88% and 75% respectively, but no anti-nuclear antibody was detected in healthy controls. PDCD5 levels in peripheral blood of patients with autoimmune diseases and healthy controls showed a significant difference (P <0.05) in PDCD5 levels between patients with SLE, RA, ITP and SS, and highest levels of PDCD5 in patients with SLE. Conclusion: There is abnormal expression of PDCD5 in patients with autoimmune diseases, and the detection of antinuclear antibody spectrum may have a certain prompt effect on autoimmune diseases.