论文部分内容阅读
自Schild等人(1975)首次用凝胶溶血试验(HIG)测定抗体以来,此方法引起作者们的广泛重视,相继做了较深入的研究,现已应用于多种病毒如流行性腮腺炎病毒、副流感病毒、风疹病毒、冠状病毒以及某些披膜病毒抗体的测定。一般通过血凝反应或某些化学试剂如铬或高碘酸盐偶联使病毒抗原结合到红细胞膜上。本文介绍了用森利基森林病毒(SFV)包膜蛋白融合红细胞制备凝胶溶血板测定抗体的方法。 SFV的包膜与红细胞膜融合的结果产生一种构型,此构型便于包膜蛋白与抗体起反应,从而可用于制备凝胶板以测定SFV抗体。具体方法如下:纯化的SFV和20%—
Since the first determination of antibodies by Gel Hemolysis (HIG) by Schild et al. (1975), this method has drawn much attention from authors and has been studied in more depth and has been applied to various viruses such as mumps virus , Parainfluenza virus, rubella virus, coronavirus, and certain togavirus antibodies. Viral antigens are typically bound to the erythrocyte membrane by hemagglutination or by certain chemical agents such as chromium or periodate coupling. This article describes a method for the determination of antibodies by gel permeabilization of erythrocytes prepared by fusion of neutrophils with neutrophil forest virus (SFV). As a result of the fusion of the envelope of SFV with the erythrocyte membrane, a configuration is created that allows the envelope protein to react with the antibody and thus can be used to prepare a gel plate for the determination of SFV antibodies. The specific method is as follows: purified SFV and 20%