论文部分内容阅读
诱导多能干细胞(induced pluripotent stem cells,i PSCs)在体外可被诱导分化为多种细胞,该项技术在细胞治疗、药物筛选及疾病研究上具有广阔的前景。体外定向诱导其向造血分化可为临床上使用的造血干细胞提供一种新的来源,提高i PSCs的造血分化效率将是i PSCs临床前治疗要解决的关键问题。该研究采用非整合型病毒重编程正常人的外周血来源的单个核细胞(peripheral blood-derived mononuclear cells,PBMCs),诱导生成i PSCs后对其进行体外造血分化实验。结果显示,通过此种方法进行重编程的i PSCs可稳定传代,体内外均可向三胚层分化。使用OP9细胞与i PSCs共培养可分化为造血干/祖细胞,且添加细胞因子可有效提高分化效率。该研究为进一步提高i PSCs造血分化效率提供了重要的实验依据。
Induced pluripotent stem cells (iPSCs) can be induced to differentiate into many kinds of cells in vitro. This technique has broad prospects in cell therapy, drug screening and disease research. In vitro induction of hematopoietic differentiation can provide a new source of hematopoietic stem cells for clinical use. Increasing the efficiency of hematopoietic differentiation of iPSCs will be a key issue to be solved in preclinical treatment of iPSCs. In this study, peripheral blood-derived mononuclear cells (PBMCs) from normal individuals were reprogrammed by non-integrated virus to induce iPSCs to undergo in vitro hematopoietic differentiation. The results showed that iPSCs reprogrammed by this method can be stably passaged and can differentiate into the three germ layers both in vitro and in vivo. The co-culture of OP9 cells with iPSCs can differentiate into hematopoietic stem / progenitor cells, and the addition of cytokines can effectively improve the differentiation efficiency. This study provides important experimental evidence for further improving hematopoietic differentiation efficiency of iPSCs.