以东海稽稻(Oryza sativa L)为材料,研究了试管苗在最低量生长条件下长期保存的适宜条件。试管苗在含BA0.5mg/1和ABA5mg/1的培养基上,不经转移,保存了一年,移栽入土后生长基本正常。剑叶的酯酶同功酶酶谱不变,但多数农艺性状不如实生植株。种子后代与对照无显著差异。应用本方法,已成功地将普通野生稻(Oryza sa-tiva L.F.spontanea,Roschev)的腋芽在试管中保存了13个月,並移栽成活。讨论了影响试管苗长期保存的若干因素和长期保存后判断种质稳定性的指标。 Using Oryza sativa L as material, suitable conditions for long-term storage of test-tube seedlings under minimum growth conditions were studied. The test-tube seedlings were stored for one year in medium containing BA 0.5 mg / 1 and ABA 5 mg / 1 without any metastasis, and their growth was basically normal after transplanting into the soil. Esterase isozyme zymogram unchanged, but most agronomic traits as real plants. There was no significant difference between seed progeny and control. Using this method, the axillary buds of Oryza sativa L. F.spontanea (Roschev) have been successfully preserved in vitro for 13 months and transplanted to survive. Several factors that affect the long-term conservation of test-tube seedlings and the indicators of germplasm stability after long-term preservation are discussed.