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目的:优化大孔树脂分离纯化巴豆中木兰花碱的工艺条件。方法:采用HPLC法测定木兰花碱,筛选AB-8,HPD400,ADS-17,NKA-9,D101树脂对木兰花碱的吸附和解吸附能力,并优化分离纯化条件。结果:优选出D101树脂,上样液浓度为生药1.5g/mL,吸附流速1.5BV/h,药材量:树脂量为6∶1;分离纯化条件为:先用4BV水冲洗除去不吸附杂质,再用3BV 8%乙醇除去大极性干扰杂质,最后用4BV 23%乙醇冲洗收集目标滤液。结论:该方法简便易行,适用于巴豆中木兰花碱的精制与纯化。
Objective: To optimize the conditions for the separation and purification of magnolol from croton in macroporous resin. METHODS: Magnoliaine was determined by HPLC. The adsorption and desorption capacities of magnolol were screened by AB-8, HPD400, ADS-17, NKA-9 and D101 resins, and the purification conditions were optimized. Results: D101 resin was optimal. The concentration of crude drug was 1.5g / mL for crude drug and the flow rate was 1.5BV / h. The amount of drug: resin was 6:1. The separation and purification conditions were as follows: Then remove the large polar interference impurities with 3BV 8% ethanol, and finally rinse with 4BV 23% ethanol to collect the target filtrate. Conclusion: The method is simple and easy to use and is suitable for the purification and purification of Magnoliaceae in Croton.