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目的探讨太白银莲花皂苷6对U87胶质母细胞瘤细胞增殖的影响及可能机制。方法用(0.0、1.6、3.2、6.4、12.8)μg/m L太白银莲花皂苷6处理U87细胞24h、48h,采用MTT法检测太白银莲花皂苷6对细胞增殖的抑制作用;annexinⅤ-FITC/PI双染色结合流式细胞术检测细胞凋亡;Western blot法检测活化的caspase-3蛋白表达。结果与对照组相比,太白银莲花皂苷6能明显抑制U87细胞增殖,并且呈时间、剂量依赖性;随着药物浓度增加,U87细胞的凋亡率增加,活化caspase-3蛋白表达增强。结论太白银莲花皂苷6可呈剂量依赖性抑制U87细胞增殖,增加活化caspase-3蛋白表达,促进凋亡。
Objective To investigate the effect of Pulsatilla esculenta 6 on the proliferation of U87 glioblastoma cells and its possible mechanism. Methods U87 cells were treated with (0.0, 1.6, 3.2, 6.4, 12.8) μg / m L of the white lotus flower saponin 6 24h, 48h, the inhibition of cell proliferation was detected by MTT assay; Cell apoptosis was detected by double staining combined with flow cytometry. The expression of activated caspase-3 protein was detected by Western blot. Results Compared with the control group, the proliferation of U87 cells was significantly inhibited by Taibai Yinhua saponin 6 in a time-and dose-dependent manner. With the increase of drug concentration, the apoptosis rate of U87 cells increased and the expression of activated caspase-3 protein increased. Conclusion Taibai Yinhua saponin 6 can inhibit the proliferation of U87 cells in a dose-dependent manner, increase the expression of activated caspase-3 and promote apoptosis.