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目的观察白内障患者房水与血清中白介素-17(IL-17)、基质金属蛋白酶-2(MMP-2)的浓度,探讨其在白内障发病过程中的临床意义。方法选择2016年1月至2017年1月我院收治的白内障患者90例为患者组,选择同期来我院体检的健康志愿者80例为对照组,运用酶联免疫吸附法(ELISA)检测两组受试对象血清与房水中IL-17和MMP-2水平,并对白内障患者血清及房水IL-17和MMP-2水平实施相关性分析。结果患者组治疗前血清IL-17含量为(27.85±3.72)ng/l,明显高于对照组血清IL-17含量(5.03±0.24)ng/l(P<0.05);患者组治疗前血清MMP-2含量为(12.36±2.42)pg/ml,明显高于对照组血清MMP-2含量(3.97±0.35)pg/ml(P<0.05)。患者组治疗前房水IL-17含量为(18.62±2.51)ng/l,明显高于对照组房水IL-17含量(2.12±0.65)ng/l(P<0.05);患者组治疗前房水MMP-2含量为(9.44±1.32)pg/ml,明显高于对照组房水MMP-2含量(1.37±0.25)pg/ml(P<0.05)。经Pearson相关分析显示,患者组白内障患者血清中IL-17与MMP-2水平呈正相关(r=0.736,P<0.05),患者组白内障患者房水中IL-17与MMP-2水平呈正相关(r=0.704,P<0.05)。结论 IL-17和MMP-2在白内障患者房水与血清中存在高表达,其可能跟此病患者眼内炎症反应紧密相关,参与白内障的发生及发展。
Objective To observe the concentrations of interleukin-17 (IL-17) and matrix metalloproteinase-2 (MMP-2) in aqueous humor and serum of patients with cataract and to explore their clinical significance in the pathogenesis of cataract. Methods Ninety patients with cataract treated in our hospital from January 2016 to January 2017 were selected as the control group. Eighty healthy volunteers who were checked up in our hospital during the same period were selected as the control group. Enzyme-linked immunosorbent assay (ELISA) The levels of IL-17 and MMP-2 in the serum and aqueous humor of the subjects were analyzed. Correlation analysis was made between the levels of IL-17 and MMP-2 in serum and aqueous humor of cataract patients. Results The serum level of IL-17 in patients before treatment was (27.85 ± 3.72) ng / l, which was significantly higher than that in control group (5.03 ± 0.24) ng / l (P <0.05) -2 was (12.36 ± 2.42) pg / ml, which was significantly higher than that of the control group (3.97 ± 0.35) pg / ml (P <0.05). The level of IL-17 in aqueous humor before treatment in patients group was (18.62 ± 2.51) ng / l, which was significantly higher than that in control group (2.12 ± 0.65) ng / l (P <0.05) The level of MMP-2 in water was (9.44 ± 1.32) pg / ml, which was significantly higher than that in control group (1.37 ± 0.25) pg / ml (P <0.05). Pearson correlation analysis showed that there was a positive correlation between serum IL-17 and MMP-2 levels in patients with cataract (r = 0.736, P <0.05). There was a positive correlation between IL-17 and MMP-2 in atrophic cataract patients = 0.704, P <0.05). Conclusion IL-17 and MMP-2 are highly expressed in the aqueous humor and serum of patients with cataract, which may be closely related to intraocular inflammation in patients with cataract and participate in the occurrence and development of cataract.