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目的 探讨散发性大肠癌与APC基因突变的关系。方法 41例大肠癌组织标本 ,应用银染 单链构相多态性技术分析APC基因突变情况。结果 41例大肠癌患者组织标本中 ,APC基因突变率为 48 8% ( 2 0 41例 )。引物 1、引物 2组织标本扩增产物突变检出率分别为 15 41( 36 6% )、7 41( 17 1% ) ,两者同时检测到突变的有 2例。结果还表明 ,APC基因的突变与CEA水平、淋巴结转移、病理分化程度统计学上没有显著性关系。结论 APC基因突变是散发性大肠癌较常见的基因改变事件。进行多位点突变检测可以提高突变的检出率。实验还表明 ,APC基因突变可能是大肠癌发生、发展中的独立事件
Objective To investigate the relationship between sporadic colorectal cancer and APC gene mutation. Methods Forty-one cases of colorectal cancer tissue samples were analyzed for APC gene mutation using silver-stained single-stranded constitutive polymorphism. Results Among 41 specimens of colorectal cancer patients, the APC gene mutation rate was 488% (204 cases). The detection rates of amplification products of primers 1 and 2 of tissue specimens were 15 41 (36 6%) and 7 41 (17 1%), respectively. There were two cases of mutation detected at the same time. The results also showed that there was no statistically significant relationship between APC gene mutations and CEA levels, lymph node metastasis, and pathological differentiation. Conclusion APC gene mutation is a common gene mutation event in sporadic colorectal cancer. Detection of multiple site mutations can increase the detection rate of mutations. The experiment also showed that APC gene mutation may be an independent event in the occurrence and development of colorectal cancer.