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目的 研究非毒性分化诱导剂苯乙酸钠 (Na PA)在体外对胃癌 MKN- 2 8和 MKN- 4 5细胞的生长、分化以及表型的影响。方法 以 3 H- Td R掺入法和半固体琼脂培养法分别检测了 MKN- 2 8和 MKN- 4 5细胞的着壁依赖性和着壁不依赖性生长。以酶联吸附试验和流式细胞仪分别检测了 MKN- 2 8和 MKN- 4 5细胞表面人白细胞抗原 、 类分子以及细胞间粘附分子 - 1的表达。以电子显微镜观察了 MKN- 2 8和 MKN- 4 5细胞的超微结构。结果 Na PA能够抑制 MKN- 2 8和 MKN- 4 5细胞的着壁依赖性和着壁不依赖性生长 ,而对正常羊膜组织 Wish细胞和成人肝细胞 L- 0 2的生长无显著抑制作用。 Na PA能够增强 MKN- 2 8和 MKN- 4 5细胞表面人白细胞抗原 、 类分子和 MKN- 2 8细胞表面的细胞间粘附分子 - 1表达。以电子显微镜观察到 :在 Na PA未处理的 MKN- 2 8和 MKN - 4 5细胞中 ,具有大量游离的胞浆多聚核糖体 ,而在 Na PA处理的 MKN - 2 8和MKN- 4 5细胞中 ,具有丰富的线粒体、粗面内质网 ,游离的胞浆多聚核糖体减少。结论 Na PA不仅能选择性抑制胃癌细胞的生长 ,而且能有效地诱导胃癌细胞的分化及表型逆转 ,为分化治疗胃癌提供了实验依据
Objective To investigate the effect of NaPA, a non-toxic differentiation inducer, on the growth, differentiation and phenotype of gastric cancer MKN-88 and MKN-45 cells. Methods The wall-dependent and wall-independent growth of MKN-28 and MKN-45 cells were detected by 3 H-Td R incorporation and semi-solid agar culture. The expression of human leukocyte antigen, molecules and intercellular adhesion molecule-1 on the surface of MKN-28 and MKN-45 cells were detected by enzyme-linked adsorption assay and flow cytometry, respectively. The ultrastructure of MKN-28 and MKN-45 cells was observed with an electron microscope. Results Na PA could inhibit the wall-dependent and wall-independent growth of MKN- 28 and MKN-45 cells, while it had no significant inhibitory effect on the growth of normal amniotic tissue Wish cells and adult liver cells L-02. Na PA can enhance the expression of ICAM-1 on the surface of human leukocyte antigens, molecules and MKN-8 cells on the surface of MKN-8 and MKN-4 cells. Observed by electron microscopy: There were a large number of free cytosolic polyribosomes in NaPA-untreated MKN-8 and MKN-4 cells, while MKN-28 and MKN-45 in NaPA-treated cells. In cells, there are abundant mitochondria, rough endoplasmic reticulum, and free cytoplasmic polyribosomes. Conclusion Na PA can not only selectively inhibit the growth of gastric cancer cells, but also can effectively induce the differentiation and phenotype reversion of gastric cancer cells, providing an experimental basis for differentiation and treatment of gastric cancer.